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. 2026 Jan 1;16(6):2952–2983. doi: 10.7150/thno.124189

Figure 9.

Figure 9

In vivo immune analysis in tumor and lymph nodes. (A) Scatter plot of immune response-related gene expression in tumor cancer cells (MCT-NE#9—oral administration ATV 2.5 mg/kg + DTX 10 mg/kg once daily vs. MTD—IV administration of ATV 2.5 mg/kg + DTX 10 mg/kg once every 3 weeks). Key genes (Arg1, CD274, and Ifng) involved in immune regulation are highlighted (n = 3). (B) Heatmap showing the expression patterns of immune-related genes across control, MTD, and MCT-NE#9 groups. Red indicates upregulation and blue indicates downregulation of key immune markers (n = 3). Flow cytometry analysis of immune cell populations in tumor tissues and TDLNs. (C) Quantification of T-cell activation in the tumor. (D) Quantification of Tregs in the tumor. (E) Quantification of M2 macrophages in the tumor. (F) Quantification of MDSCs in the tumor. (G) Quantification of NK cells in the tumor. (H) Quantification of M1 macrophages in the tumor. (I) M1/M2 macrophage ratio in the tumor. (J) Quantification of CD4 T cells in TDLNs. (K) Quantification of CD8 T cells in TDLNs. (L) Quantification of NK cells in TDLNs. (M) Quantification of NKT cells in TDLNs. Data are presented as the mean ± SEM (n = 10). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.