FIG. 2.

Western blot analysis of S. agalactiae outer surface proteins probed with preimmune (PI), anti-phosphoglycerate kinase (PGK), and anti-ornithine carbamoyltransferase (OCT) antisera. Outer surface proteins were analyzed by SDS-polyacrylamide gel electrophoresis (Criterion 22%; Bio-Rad), blotted to nitrocellulose, and probed with a 1:5,000 dilution of preimmune-phase sera or antisera. Blots were decorated with anti-rabbit alkaline phosphatase conjugate secondary antibody and were developed with Western blue substrate (Promega). Mw, molecular weight.