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. 2002 Mar;70(3):1254–1259. doi: 10.1128/IAI.70.3.1254-1259.2002

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Copyright © 2002, American Society for Microbiology.

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FIG. 3. — Sera directed against S. agalactiae phosphoglycerate kinase (PGK) and ornithine carbamoyltransferase (OCT) recognize S. agalactiae in a whole-cell ELISA. Cells of six S. agalactiae serotypes, type Ia/c (RF76a), type Ib/c (H36b), type II (18RS21, type III (M732), type IV (R80405), and type V (Prague strain), were used to coat ELISA plates which were subsequently challenged with rabbit preimmune sera or antisera raised against PGK or OCT. The reacting antibody was decorated with secondary (goat anti-rabbit, alkaline phosphatase-conjugated) antibody, and the reaction was developed with p-nitrophenyl phosphate and read at 405 nm. OD405, optical density at 405 nm.