TABLE 2.

Peptide sequences and protein identification/assignation from proteins derived from two-dimensional gel separation of S. agaldctiae outer surface proteins^a

Spot no.	Peptide sequence	Identification
15	AIGLVIPELNGK	Glyceraldehyde-3-phosphate dehydrogenase
	IQNVEGVEVTR
16	AIGLVIPELNGK	Glyceraldehyde-3-phosphate dehydrogenase
	IQNVEGVEVTR
17	IQNVEGVEVTR	Glyceraldehyde-3-phosphate dehydrogenase
18	PAFHD	Ornithine carbamoyltransferase
	HFQDA
19	FPAEE(I/L)VK	Ornithine carbamoyltransferase
	EVTD
20	YNQ(I/L)(I/L)R	Enolase
	FEGTEDRE
	ETAVG
21	VVEEVADE(L/I)AEK	Nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase
	GPDNFPF(L/I)G(L/I)K
22	(L/I)GQDVVF	Phosphoglycerate kinase
	(L/I)GGGDSAAA	Phosphoglycerate kinase
23	(I/L)A(I/L)AM(I/L)ED	Purine nucleoside phosphatase
	DAF(I/L)AGVGT	Purine nucleoside phosphatase

^aAssignation of proteins was possible only after reverse genetic analysis. The peptide sequences shown were used to synthesize oligonucleotides, which were in turn used for PCR amplification of the gene from S. agalactiae genomic DNA. The DNA sequence was used to search the database and assign the protein identification.