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. 2002 Mar;70(3):1507–1517. doi: 10.1128/IAI.70.3.1507-1517.2002

FIG. 10.

FIG. 10.

Antibodies elicited by i.n. immunization with PAO1ΔaroA are directed against the LPS of P. aeruginosa. (A) IgG titers of mouse and rabbit antisera to PAO1ΔaroA against P. aeruginosa PAO1, AK44 (an O-antigen-deficient, complete-LPS-core mutant of PAO1), and AK1012 (an O-antigen-deficient, incomplete-LPS-core mutant of PAO1). Bars represent endpoint titers as determined by linear regression, and error bars indicate the 95% confidence intervals. (B) Adsorption of rabbit antiserum to PAO1ΔaroA with the O-antigen-deficient mutants AK44 and PAC557 does not alter the opsonic activity, while adsorption with PAO1 abolishes opsonic activity. Bars represent mean percent killing of two to four replicates relative to no-serum control, and error bars represent the standard errors of the means. Negative killing indicates growth during the 90-min incubation. Tubes with the variously adsorbed PAO1ΔaroA antisera but without PMNs served as negative controls. ∗, P < 0.0001 by ANOVA with Fisher's PLSD for comparisons with either unadsorbed, AK44-adsorbed, or PAC557-adsorbed antiserum.