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. 2002 Mar;70(3):1507–1517. doi: 10.1128/IAI.70.3.1507-1517.2002

FIG. 7.

FIG. 7.

Opsonophagocytic killing of P. aeruginosa serogroup O2/O5 (A) and heterologous (B) strains by a 1:8 dilution of antisera from PAO1ΔaroA-immunized or E. coli HB101-immunized (control) C3H/HeN mice. The International Antigenic Typing Scheme (IATS) (31, 32) serogroup of each heterologous strain is indicated below the strain designation. Bars represent mean percent killing of two to four replicates relative to no-serum control, and error bars represent the standard errors of the means. Negative killing indicates growth during the 90-min incubation. Rabbit antisera raised by intravenous immunization with heat-killed whole bacteria from the same serogroup served as positive controls. Tubes with PAO1ΔaroA antiserum but without PMNs served as additional negative controls. ∗, P < 0.001; #, P < 0.05 by ANOVA with Fisher's PLSD for comparison with E. coli HB101 antiserum., P < 0.01 by ANOVA with Fisher's PLSD for comparison with PAO1ΔaroA antiserum.