TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2d | M. Vasil |
| PAO1ΔaroA | aroA deletion mutant of PAO1, LPS smooth, serogroup O2/O5, subtype epitopes O2a,O2d | This study |
| PAO1ΔaroA (pUCP18) | PAO1ΔaroA containing the empty vector pUCP18, CbR | This study |
| PAO1ΔaroA (pMB1) | PAO1ΔaroA containing the intact aroA gene on plasmid pMB1, CbR | This study |
| AK44 | LPS-defective derivative of PAO1 (absent O antigen, complete outer core) | 30 |
| AK1012 | LPS-defective derivative of PAO1 (absent O antigen, incomplete core) | 25 |
| PAC1R | Wild-type strain, LPS smooth, serogroup O3 | 53 |
| PAC557 | LPS-rough derivative of PAC1R (absent O antigen, complete outer core) | 53 |
| 170003 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2b | 19 |
| IATS O16 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2b, O2e | 19 |
| Fisher IT-3 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2c | 19 |
| 170006 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2d, O2e | 19 |
| 170007 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitopes O2a, O2d, O2f | 19 |
| Fisher IT-7 | Wild-type strain, LPS smooth, serogroup O2/O5, subtype epitope O2a | 19 |
| Fisher IT-4 | Clinical isolate (bacteremia) designated Rhodes, LPS smooth, serogroup O1 | 50 |
| 170001 | Wild-type strain, LPS smooth, serogroup O3, subtype epitopes O3a, O3b | 27, 28 |
| IATS O3 | Wild-type strain, LPS smooth, serogroup O3, subtype epitopes O3a, O3b, O3c | 27, 28 |
| 6294 | Clinical isolate (corneal infection), LPS smooth, noncytotoxic, serogroup O6 | BPEIb; 15, 50 |
| 6073 | Clinical isolate (corneal infection), LPS smooth, cytotoxic, serogroup O11 | BPEI; 15, 50 |
| 6077 | Clinical isolate (corneal infection), LPS smooth, cytotoxic, serogroup O11 | BPEI; 15, 50 |
| 6206 | Clinical isolate (corneal infection), LPS smooth, cytotoxic, serogroup O11 | BPEI; 15, 50 |
| E. coli | ||
| HB101 | supE44 hsdS20(r−Bm−B) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1, | 2 |
| S17-1 | thi pro hsdR recA RP4-2-Tc::Mu Km::Tn7 | 57 |
| SM10 | thi-1, thr leu tonA lacY supE recA::RP4-2-Tc::Mu | 7 |
| Plasmids | ||
| pMB1 | pUCP18-based plasmid containing the intact aroA gene, ApR | This study |
| pUCP18 | Broad host range shuttle vector, ApR | 55 |
| pMB4 | Derivative of pMB1 containing the aroA gene disrupted by the aacC1 cassette from pPS856, ApR/GmR | This study |
| pEX100T | oriT+sacB+ gene-replacement vector | 23, 56 |
| pMB11 | pEX 100T-based derivative of pMB4 containing the sacB sucrose-sensitivity gene, CbR/GmR/sucS | This study; 23 |
| pPS856 | Plasmid containing the aacC1 gentamicin-resistance cassette flanked by the Flp-recombinase target sequences, ApR/GmR | 23 |
| pFLP2 | Plasmid containing the Flp recombinase, ApR | 23 |
a
Abbreviations: Ap, ampicillin; Gm, gentamicin; Cb, carbenicillin; Km, kanamycin; suc, sucrose; Tc, tetracycline; superscript R, resistant; superscript S, sensitive.
b
BPEI, Bascom-Palmer Eye Institute, Miami, Fla.