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Reactivity of MAbs with denatured or native CTB. MAb reactivity was determined by direct-SPRIA, S-SPRIA, and GM1-SPRIA. For panels A and C, reactivity was determined against serial dilutions of native CTB (starting at 0.5 μg/ml). MAb culture supernatants were used at a 1/5 dilution for 12D2 and 1E9 and at a 1/30 dilution for 32D3. MAbs were titrated by GM1-SPRIA (B) with CTB at 0.125 μg/ml and by direct-SPRIA (D) with CTB at 1 μg/ml denatured by boiling for 10 min.
