TABLE 3.

CTB variants affecting epitopes of anti-CTB MAbs

Wild-type CTB residue	Resulta with MAb:
	1E9	4C8	4E2	32D3	15C11	22C6	40B10	40D9	35G8
Ala-10	(A10V)	(A10V)	(A10V)	A10V	A10V	(A10V)	(A10V)	(A10V)	+
Glu-11	+	E11K	+	+	+	+	+	+	+
Tyr-12	+	Y12D	+	+	+	+	Y12D	+	+
Gly-33	+	(G33∗)	+	+	+	+	G33∗	+	+
Lys-34	+	K34∗	+	+	+	+	K34∗	+	+
Arg-35	+	R35∗	+	+	+	+	(R35∗)	+	+
Glu-36	+	E36Q	+	+	+	+	(E36Q)	+	+
Ala-46	(A46V)	+	(A46V)	+	A46V	A46V	+	A46V	A46V
Glu-51	+	(E51K)	+	+	(E51K)	+	E51K	+	+
Trp-88	+	(W88∗)	+	+	+	+	(W88∗)	+	+
Lys-91	ND	+	+	+	(K91D)	+	(K91D)	+	+
Ala-95	(A95D)	(A95D)	+	+	+	+	(A95D)	+	+

^aSubstitutions shown abolished reactivity with the MAb. Parentheses indicate partial reactivity retained. A plus sign indicates that the MAb retains full reactivity with all variants tested. An asterisk indicates more than one substitution tested affects reactivity. ND, not done.