TABLE 3.
Wild-type CTB residue | Resulta with MAb:
|
||||||||
---|---|---|---|---|---|---|---|---|---|
1E9 | 4C8 | 4E2 | 32D3 | 15C11 | 22C6 | 40B10 | 40D9 | 35G8 | |
Ala-10 | (A10V) | (A10V) | (A10V) | A10V | A10V | (A10V) | (A10V) | (A10V) | + |
Glu-11 | + | E11K | + | + | + | + | + | + | + |
Tyr-12 | + | Y12D | + | + | + | + | Y12D | + | + |
Gly-33 | + | (G33∗) | + | + | + | + | G33∗ | + | + |
Lys-34 | + | K34∗ | + | + | + | + | K34∗ | + | + |
Arg-35 | + | R35∗ | + | + | + | + | (R35∗) | + | + |
Glu-36 | + | E36Q | + | + | + | + | (E36Q) | + | + |
Ala-46 | (A46V) | + | (A46V) | + | A46V | A46V | + | A46V | A46V |
Glu-51 | + | (E51K) | + | + | (E51K) | + | E51K | + | + |
Trp-88 | + | (W88∗) | + | + | + | + | (W88∗) | + | + |
Lys-91 | ND | + | + | + | (K91D) | + | (K91D) | + | + |
Ala-95 | (A95D) | (A95D) | + | + | + | + | (A95D) | + | + |
Substitutions shown abolished reactivity with the MAb. Parentheses indicate partial reactivity retained. A plus sign indicates that the MAb retains full reactivity with all variants tested. An asterisk indicates more than one substitution tested affects reactivity. ND, not done.