TABLE 3.
Cytokine levels in PBMC from individuals from the high and low transmission villages in response to stimulation with adult L. loa Agc
| Ag | IL-2 (U/ml)
|
IFN-γ (pg/ml)
|
IL-4 (pg/ml)
|
IL-5 (pg/ml)
|
||||
|---|---|---|---|---|---|---|---|---|
| Low | High | Low | High | Low | High | Low | High | |
| L3 | 0 | 13.9 ± 10.1 | 8.12 ± 5.82 | 67.4 ± 23.2 | 0a | 0a | 65 ± 60.8 | 40.8 ± 17 |
| Adult | 32.5 ± 23.3 | 65 ± 35.8 | 50 ± 25.8 | 121.3 ± 45.8 | 7.5 ± 4.91b | 26.3 ± 10.2 | 616 ± 437 | 802 ± 291 |
| Mf | 112.5 ± 85.4 | 130.5 ± 51.5 | 51.9 ± 28.5 | 138.7 ± 57 | 20 ± 16.7 | 48.4 ± 16.4 | 848 ± 497 | 1,251 ± 466 |
a
A single individual in each group secreted IL-4 over background levels.
b
Only three individuals secreted IL-4 at levels above the cutoff (10 pg/ml).
c
In the high transmission village, IL-4 levels were significantly higher with adult Ag versus L3 (P = 0.0017) and with Mf Ag versus L3 (P = 0.0015); IL-5 levels were significantly higher with adult Ag versus L3 (P = 0.0025) and with Mf Ag versus L3 (P = 0.0008). In the low transmission village, IL-5 levels were significantly higher with Mf Ag versus L3 (P = 0.0101). Sensitivity was 10 pg/ml for IL-4 and IL-5, 7.8 pg/ml for IFN-γ, and 0.3 U/ml for IL-2.