TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| J96 | Clinical isolate | 28 |
| NU149 | Clinical isolate | 30 |
| NU14 | Clinical isolate | 31 |
| NU2-25 | Clinical isolate | 31 |
| NU2-38 | Clinical isolate | 31 |
| NU2-39 | Clinical isolate | 31 |
| AAEC189 | YMC9 Δfim operon Δlac recA endA | 5 |
| DH5α | supE44 ΔlacU169(lacZΔM15) hsdR17 endA1 gyrA96 thi-1 relA1 | Gibco/BRL |
| MC4100 | F− Δ(lac)U169 araD139 rpsL relA thiA flbB | Linda Kenney |
| MH1160 | MC4100 ompB101 (ompR101) | Linda Kenney |
| RR1 | supE44 lacY1 hsdS20 ara-19 proA2 galK2 rpsL20 xyl-5 mtl-1 | Staffan Normark |
| RR1 bglY | RR1 bglY (hns) | Staffan Normark |
| AAEC189-4fimB | AAEC189 chromosomal fimB-lacZ integration | This study |
| AAEC189-2fimE | AAEC189 chromosomal fimE-lacZ integration | This study |
| Plasmids | ||
| pPR274 | Mini-F replicon Cmr | 48 |
| pPP2-6 | pPR274 with MCSa | James Duncan (Brenda Bloom) |
| pBB2-1 | fimA-lacZYA on pPR274 | James Duncan (Magaret Pearle) |
| pUTE1 | fimE-lacZYA on pUT-Tc | James Duncan (Margaret Pearle) |
| pP5-48 | fimB-lacZYA on pUJ8 | James Duncan (Margaret Pearle) |
| pAON-1 | fimA-lacZYA locked on on pUJ8 | James Duncan (Margaret Pearle) |
| pUJ8 | trp′-′lacZ phoA Apr | 10 |
| pUT-Tc | Mini-Tn5 on pUT Tcr | 10 |
| pJB5A | fimB-lacZYA on pPP2-6 | This study |
| pJLE4-3 | fimE-lacZYA on pPP2-6 | This study |
| pUTE4 | fimB-lacZYA on pUT-Tc | James Duncan (Margaret Pearle) |
| pWS124-17 | fimA-lacZYA locked on on pPP2-6 | This study |
a
Multiple cloning sites.