TABLE 3.

EIAs and HA assays with type 1 piliated bacteria grown in pH 7.0 and 5.5 broth media under low- or high-osmolarity conditions

Strain	Agar		pH 7.0 broth				pH 5.5 broth
			No NaCl added		400 mM NaCl added		No NaCl added		400 mM NaCl added
	EIAa	HA assayb	EIA	HA assay	EIA	HA assay	EIA	HA assay	EIA	HA assay
NU149	0.15 ± 0.01	0	2.56 ± 0.23	512	1.84 ± 0.16	128	1.40 ± 0.01	64	0.60 ± 0.04	24
J96	0.16 ± 0.02	0	2.76 ± 0.18	1,024	2.03 ± 0.08	256	1.77 ± 0.52	96	0.86 ± 0.06	48
NU14	0.67 ± 0.01	32	2.52 ± 0.17	512	1.81 ± 0.27	196	1.47 ± 0.20	96	0.76 ± 0.06	48
NU2-25	0.16 ± 0.01	0	2.11 ± 0.15	196	1.68 ± 0.09	128	1.60 ± 0.12	64	0.51 ± 0.04	16
NU2-38	0.22 ± 0.01	0	2.33 ± 0.16	512	2.05 ± 0.06	196	1.60 ± 0.08	64	0.57 ± 0.06	24
NU2-39	0.17 ± 0.02	0	1.88 ± 0.05	128	0.89 ± 0.04	64	0.63 ± 0.07	12	0.24 ± 0.14	±c

^aEIA data are expressed in optical density at 492 nm; the values are means ± standard deviations based on at least two separate experiments.

^bThe HA assay data are average HA titers based on at least three separate experiments.

^c±, weak reaction; the first well showed some agglutination.