TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− φ80d lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) phoA supE44λ−thi-1 gyrA96 relA1 | GIBCO BRL |
| B. pertussis strains | ||
| BP536 | Wild-type, nalidixic acid-resistant, streptomycin-resistant derivative of Tohama I | 38 |
| BP536Δptx | BP536 with a 2.6-kb deletion in the ptx genes from nucleotide 936 to nucleotide 3514 | 16 |
| BP536Δptxptl | BP536 with an 11.5-kb deletion in the ptx-ptl genes from nucleotide 425 to nucleotide 11971 | 16 |
| Plasmids | ||
| pTH18 | pUFR047 containing ptxS2, ptxS4,ptxS5, and ptxS3 under lacZ promoter control | 16 |
| pTH19 | pRK415 containing the ptx-ptl promoter region and ptxS1 | 16 |
| pDMC36 | pUFR047 containing the ptx-ptl promoter region and ptxS1 | 16 |
| pAMC61 | pUFR047 containing the ptx-ptl promoter region and mutant ptxS1 with a deletion in the coding sequence for Phe235 | This study |
| pSZH4 | pUC19 containing the entire ptx-ptl region | 19 |
| pUFR047 | Broad-host-range (IncW) vector, Mob+lacZα+, gentamicin resistant | 13 |
| pRK415 | Broad-host-range (IncP1) vector, tetracycline resistant | 25 |
| pRK2013 | ColE1 Tra+ kanamycin-resistant helper plasmid | 17 |
| pGEM11Zf(+) | Ampicillin resistant cloning vector | Promega |
| pAlterEx1 | Tetracycline-resistant cloning vector | Promega |
| pUC19 | Ampicillin-resistant cloning vector | GIBCO BRL |