Table 2.

Non-unique nearest-neighbor thermodynamics of inosine mismatches in 1 M NaCl

Dimer sequence	$\Delta G_{37}^{°}$ (kcal/mol)	ΔH° (kcal/mol)	ΔS° (cal/K mol)
I·C pairs
AI/TC	−0.96 ± 0.06	−8.9 ± 1.4	−25.5 ± 1.4
TI/AC	−0.46 ± 0.06	−5.9 ± 1.5	−17.4 ± 1.5
AC/TI	−0.89 ± 0.06	−8.8 ± 1.4	−25.4 ± 1.4
TC/AI	−0.59 ± 0.07	−4.9 ± 1.6	−13.9 ± 1.6
CI/GC	−1.14 ± 0.08	−5.4 ± 1.6	−13.7 ± 1.6
GI/CC	−0.86 ± 0.08	−6.8 ± 1.6	−19.1 ± 1.6
CC/GI	−0.88 ± 0.09	−8.3 ± 1.7	−23.8 ± 1.7
GC/CI	−1.07 ± 0.08	−5.0 ± 1.7	−12.6 ± 1.7
I·A pairs
AI/TA	−0.51 ± 0.06	−8.3 ± 1.5	−25.0 ± 1.5
TI/AA	0.09 ± 0.06	−3.4 ± 1.4	−11.2 ± 1.4
AA/TI	0.12 ± 0.07	−0.7 ± 1.7	−2.6 ± 1.7
TA/AI	0.12 ± 0.06	−1.3 ± 1.4	−4.6 ± 1.4
CI/GA	−0.18 ± 0.08	2.6 ± 1.6	8.9 ± 1.6
GI/CA	−1.24 ± 0.08	−7.8 ± 1.7	−21.1 ± 1.7
CA/GI	−0.77 ± 0.07	−7.0 ± 1.6	−20.0 ± 1.6
GA/CI	−1.33 ± 0.08	−7.6 ± 1.6	−20.2 ± 1.6
I·T pairs
AI/TT	0.71 ± 0.06	0.49 ± 1.5	−0.7 ± 1.4
TI/AT	0.36 ± 0.06	−6.5 ± 1.5	−22.0 ± 1.3
AT/TI	0.22 ± 0.07	−5.6 ± 1.7	−18.7 ± 1.6
TT/AI	0.54 ± 0.05	−0.8 ± 1.4	−4.3 ± 1.4
CI/GT	−0.26 ± 0.07	−1.0 ± 1.5	−2.4 ± 1.5
GI/CT	−0.19 ± 0.08	−3.5 ± 1.8	−10.6 ± 1.8
CT/GI	0.41 ± 0.07	0.1 ± 1.5	−1.0 ± 1.5
GT/CI	−0.54 ± 0.08	−4.3 ± 1.8	−12.1 ± 1.7
I·G pairs
AI/TG	0.02 ± 0.06	−4.9 ± 1.6	−15.8 ± 1.6
TI/AG	0.76 ± 0.06	−1.9 ± 1.5	−8.5 ± 1.5
AG/TI	0.65 ± 0.07	0.1 ± 1.7	−1.8 ± 1.7
TG/AI	0.70 ± 0.06	1.0 ± 1.4	1.0 ± 1.4
CI/GG	0.47 ± 0.10	7.1 ± 2.3	21.3 ± 2.3
GI/CG	−0.10 ± 0.07	−1.1 ± 1.5	−3.2 ± 1.5
CG/GI	0.54 ± 0.09	5.8 ± 2.0	16.9 ± 2.0
GG/CI	−0.74 ± 0.08	−7.6 ± 1.8	−22.0 ± 1.8
I·I pairs
AI/TI	0.40 ± 0.05	−3.3 ± 1.2	−11.9 ± 3.9
TI/AI	0.81 ± 0.05	0.1 ± 1.4	−2.3 ± 4.3
CI/GI	0.36 ± 0.06	1.3 ± 1.4	3.0 ± 4.2
GI/CI	−0.09 ± 0.06	−0.5 ± 1.2	−1.3 ± 3.7

The I·C, I·A, I·T and I·G parameters are a linear least-squares fit of the data for a singular matrix with a rank of 7. The parameters for I·I are not rank deficient. These parameters were used to make the predictions found in Table 1. These parameters are for internal Inosine pairs only (note that these parameters do not apply to terminal helix inosine pairs). Errors shown are standard deviations computed by error propagation. Underlined residues are paired.