Table 3.
The thermodynamics contribution of tandem internal Inosine-Watson–Crick mismatch pairs
| Propagation sequence | (kcal/mol) | ΔH° (kcal/mol) | ΔS° (cal/K mol) |
|---|---|---|---|
| GGTAIIGTGTCG | |||
| II/CC | −0.64 ± 0.93 | −9.3 ± 5.8 | −28.0 ± 17.1 |
| II/AC | 0.27 ± 0.25 | −3.1 ± 5.8 | −11.0 ± 16.8 |
| GGTTIIGAGTCG | |||
| II/CA | 0.44 ± 0.27 | −8.7 ± 6.2 | −29.3 ± 18.2 |
| II/AA | −0.27 ± 0.27 | −2.1 ± 5.7 | −6.1 ± 18.1 |
| II/TA | 0.83 ± 0.26 | 2.3 ± 6.1 | 4.4 ± 18.0 |
| II/GA | 0.30± 0.25 | 4.2 ± 5.8 | −14.8 ± 17.1 |
| GGTGCIIGGTCG | |||
| II/CT | 0.33 ± 0.30 | −14.5 ± 6.1 | −47.8 ± 17.5 |
| II/AT | 0.19 ± 0.30 | −17.8 ± 6.1 | −58.1 ± 17.6 |
| II/TT | 1.69 ± 0.30 | −7.0 ± 6.1 | −28.1± 17.5 |
| II/GT | 0.13 ± 0.31 | −19.4 ± 6.3 | −62.8 ± 17.7 |
| GGTTIIAGTTCG | |||
| II/TG | 0.03 ± 0.22 | 13.3 ± 6.8 | 40.6 ± 21.3 |
| II/TG | −1.30 ± 0.18 | 0.3 ± 6.3 | 2.1 ± 19.6 |
| GGTAIIGTGTCG | |||
| II/II | (−0.7 ± 0.25) | (−13.8 ± 5.6) | (−42.1 ± 17.4) |
| GGTTIIAGTTCG | |||
| II/II | (1.74 ± 0.23) | (−7.5 ± 6.2) | (−29.0 ± 19.5) |
Values in parentheses are non two-state. Values reported were calculated by subtracting Watson–Crick nearest-neighbors and inosine nearest-neighbors from the raw experimental thermodynamics seen in Supplementary Table S3. Errors shown are standard deviations determined by error propagation. Underlined residues are paired with inosine. The opposing strands (not shown) are complementary except across from inosine.