Table 6.

The effect of inosine position on hybridization thermodynamics for 5′ ATCATCGTACG 3′ paired with sequences listed

Complementary duplex	Mismatch Type	$\Delta G_{37}^{°}$ (kcal/mol)	Tm (°C) (1 × 10−4 M CT)
AGTAGCATG	Control	−9.06	48.6
AGTAGCITG	TI	−7.22	40.3
AGTAICATG	CI	−7.32	41.0
AGIAGCATG	AI	−8.01	43.7
AGTAGIATG	GI	−4.89	27.2

The calculations were done using HYTHER Module 3, which uses the nearest neighbor parameters from Table 2 and those from reference 40. Inosines are in boldface and underlined for emphasis.