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. 2005 Nov 3;33(19):6287–6295. doi: 10.1093/nar/gki939

Figure 2.

Figure 2

Inhibition of LexA binding to the recA operator by recA operator mutants. Mobility shift assays were conducted as described in Materials and Methods with purified LexA, radiolabeled recA promoter DNA (5.0 nM) and a 100-fold molar excess of competitor recA operator mutant. Base changes are indicated by position from the 5′ end of the 14 bp operator, and the base substitution relative to the recA operator sequence. Lower and upper bands correspond to unbound and LexA-bound recA promoter DNA, respectively. Lanes corresponding to no addition of competitor DNA and non-specific DNA [poly(dI–dC)] are indicated. LexA concentrations given are for the total amount of LexA.