Table 3.
Thermodynamics of LexA mutants binding to the recA operator
| LexA | Kd (nM) | ΔΔG (kJ/mol) |
|---|---|---|
| Wild-type | 4.6 | |
| Ser41Ala | 6.0 | 0.7 |
| His44Ala | 3.7 | 0.5 |
| His46Ala | >400 | |
| Ala48Gly | 22 | 3.9 |
| Arg49Ala | >400 | |
| Thr52Ala | 6.6 | 0.9 |
| Lys53Ala | 58 | 6.3 |
Thermodynamics of LexA mutants binding to the recA operator
| LexA | Kd (nM) | ΔΔG (kJ/mol) |
|---|---|---|
| Wild-type | 4.6 | |
| Ser41Ala | 6.0 | 0.7 |
| His44Ala | 3.7 | 0.5 |
| His46Ala | >400 | |
| Ala48Gly | 22 | 3.9 |
| Arg49Ala | >400 | |
| Thr52Ala | 6.6 | 0.9 |
| Lys53Ala | 58 | 6.3 |