Table 4.
Proteins with highest phosphorylation signal from proteome chip screen for Dbf2-Mob1 substrates
| ORF Name | Common Name | Slide Signal (after normalization) | MW (kDa) | # of R-3 Sites |
| YJR060W | CBF1 | 118.4224 | 39 | 3 |
| YAL051W | OAF1 | 81.8589 | 121 | 3 |
| YBR138C | HDR1 | 8.9063 | 61 | 2 |
| YOL012C | HTZ1 | 4.0738 | 14 | 1 |
| YMR165C | SMP2 | 3.2182 | 95 | 7 |
| YPL150W | 3.1545 | 100 | 16 | |
| YDR226W | ADK1 | 3.1016 | 24 | 0 |
| YMR229C | RRP5 | 2.6954 | 193 | 5 |
| YBR118W | TEF2 | 2.619 | 50 | 0 |
| YJL108C | PRM10 | 2.5802 | 41 | 0 |
| YPR091C | 2.5784 | 87 | 3 | |
| YKL168C | KKQ8 | 2.5013 | 84 | 14 |
| YNL101W | AVT4 | 2.1486 | 80 | 7 |
| YBR285W | 1.9355 | 17 | 0 | |
| YMR239C | RNT1 | 1.593 | 54 | 2 |
| YNR047W | 1.4015 | 100 | 21 | |
| YJL076W | NET1 | 1.3521 | 128 | 11 |
| YNL155W | 1.2912 | 31 | 1 | |
| YNR006W | VPS27 | 1.1429 | 71 | 4 |
| YIL135C | VHS2 | 1.116 | 48 | 11 |
| YMR184W | 1.1144 | 22 | 3 | |
| YDL220C | CDC13 | 1.0274 | 105 | 3 |
| YBR108W | 1.0216 | 93 | 7 | |
| YDL019C | OSH2 | 0.9962 | 146 | 6 |
| YOR362C | PRE10 | 0.9895 | 32 | 0 |
Of the 67 putative substrates, the 25 putative substrates with the highest relative amount of phosphorylation signal (amount of signal relative to protein expression) as listed were chosen for further study. MW: predicted molecular weight, # or R-3 Sites: number of RXXS motifs