TABLE 1.

Cytokine levels in lung homogenates of mice inoculated with LPS, PLC, or ETA^a

Cytokine	Inoculum	Mean ± SEM of cytokine level (pg/ml) at
		Control	2	6	12	24	48
TNF-α	LPS	<15	517.7 ± 94.7***	171.7 ± 12.3*	146.1 ± 23.6*	32.2 ± 17.1	<15
	PLC	<15	93.7 ± 4.6***	41.5 ± 20.8**	<15	<15	<15
	ETA	<15	<15	<15	<15	<15	<15
IL-1β	LPS	<10	670.7 ± 70.9***	678.9 ± 173.5***	710.0 ± 133.0***	631.0 ± 125.2**	240.0 ± 53.5
	PLC	<10	458.8 ± 27.8***	744.5 ± 55.5***	237.0 ± 2.9*	90.3 ± 3.3	<10
	ETA	<10	<10	<10	18.6 ± 6.7*	17.5 ± 1.9*	11.3 ± 1.4
IL-6	LPS	<20	4,408 ± 1899*	6,234 ± 1187**	4,702 ± 1087*	768 ± 294.4*	5,367 ± 976.0*
	PLC	<20	7,588 ± 1826*	6,265 ± 1346**	1,015 ± 658.2*	2,413 ± 1317*	<20
	ETA	<20	<20	83.3 ± 68.0	503.5 ± 396.3*	<20	<20
IL-18	LPS	741 ± 271	714 ± 276	797 ± 185	775 ± 86.8	937 ± 67	618 ± 51.9
	PLC	700 ± 54.2	494 ± 51.6	509 ± 26.0	564 ± 59.2	510 ± 52.8	594 ± 113.9
	ETA	825 ± 80.1	660 ± 50.3*	355 ± 60.7***	335 ± 22.3***	210 ± 12.6***	125 ± 5.9***
IFN-γ	LPS	55.9 ± 2.1	35.3 ± 2.9	51.5 ± 4.6	63.0 ± 4.8	266.1 ± 72.9**	244.9 ± 11.1**
	PLC	44.4 ± 7.4	42.8 ± 3.5	53.7 ± 7.2	68.2 ± 4.7	56.4 ± 6.3	81.6 ± 9.3
	ETA	74.4 ± 13.3	58.6 ± 18.8	76.4 ± 16.2	63.6 ± 4.6	90.9 ± 36.2	75.2 ± 8.3
MIP-1α	LPS	<15	349.0 ± 67.7***	400.3 ± 56.4***	284.1 ± 23.0***	235.8 ± 27.0***	189.6 ± 19.5**
	PLC	<15	275.1 ± 27.8***	259.1 ± 55.5***	30.0 ± 2.9	18.8 ± 3.3	<15
	ETA	<15	<15	<15	19.0 ± 6.7*	16.8 ± 3.2*	<15
MIP-2	LPS	<15	3,543 ± 1194***	1,657 ± 166*	1,169 ± 89.8*	870.5 ± 116.2*	541.6 ± 20.9
	PLC	<15	1,875 ± 77.5***	1,014 ± 272.4***	400.5 ± 38.4	270.1 ± 57.2	215.0 ± 35.9
	ETA	<15	<15	<15	163.3 ± 28.5*	172.3 ± 11.1***	145.1 ± 11.6***

^aGroups of three to five mice were inoculated with either LPS (10 μg/mouse), PLC (0.5 μg/mouse), or ETA (2μg/mouse). Control mice received vehicle. Lungs were removed at the indicated time points (in hours) and cytokine levels were measured in lung homogenates.

^b*, P < 0.05; **, P < 0.01; ***, P < 0.001 versus respective control by factorial ANOVA.