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. 2005 Nov 4;1(5):e52. doi: 10.1371/journal.pgen.0010052

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Copyright: © 2005 Elde et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation and cellular localization of the heterotetrameric AP complexes. Location of the μ subunit is indicated. TGN, trans-Golgi network.

(B) AP complex μ subunit phylogeny. The topology illustrated is the best maximum likelihood (ML) distance for μ subunits from A. thaliana (At), D. melanogaster (Dm), H. sapiens (Hs), and T. thermophila (Tt). Both ML and maximum parsimony (MP) bootstrap support values are shown at each node.

(C) Fixed, permeabilized cells expressing GFP-tagged AP-2 μ subunit (Apm2p-GFP) (from the MTT1 promoter) were labeled with anti-centrin antibody. The distribution of Apm2p-GFP is similar to that of the CHC hub (Figure 3), but the former is additionally present at the contractile vacuole pores. Bar = 5 μm.