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. 2005 Nov 4;1(5):e52. doi: 10.1371/journal.pgen.0010052

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Copyright: © 2005 Elde et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Compiled stack of confocal sections through fixed cell expressing Drp6p-GFP (driven by the MTT1 promoter) shows localization at the nuclear envelope. Bar = 10 μm.

(B) Immuno-gold visualization of Drp6p-GFP shows localization in clusters on the cytoplasmic face of the nuclear envelope (ne). Regions of heterochromatin within the macronucleus (mac) are indicated (*). Bar = 200 nm.

(C) Domain comparison of Drp1p and Drp6p and diagrams of Drp-GFP chimeras (expressed under the MTT1 promoter) indicating localization. Numbered images at right show localization patterns observed in cells expressing chimeric proteins. Bar = 10 μm. nd, not detected.

(D) Western blot of total cell lysates from Drp-GFP cells confirms stability of full-length chimeras.