Figure 1. Distribution of Procyclin mRNAs Expressed by Tsetse-Derived Trypanosomes.

RNA was isolated from the midguts and salivary glands at various days post infection and subjected to RT-PCR using generic primers for all procyclins. The products were cloned and individual clones assigned by hybridisation with a panel of oligonucleotides (see Materials and Methods). The total number of procyclin clones analysed at each time point is indicated within the chart. The standard deviations for the data from days 11–24 derive from two independent tsetse infections.