Figure 3. Effect of Procyclin 3′ UTRs on Expression of a Reporter Gene during Fly Transmission.

The coding region of GARP was fused to individual procyclin 3′ UTRs and stably integrated in place of the corresponding procyclin coding region. The percentage of trypanosomes expressing GARP in different compartments of the digestive tract and the salivary glands of the tsetse fly was determined by immunofluorescence analysis. For the sake of clarity the figures are set to the same scale. Blank spaces on the graphs indicate that no samples were taken from a particular tissue.