FIG. 1.

(A) Adsorption of Ba1 c1 to strain 197N and the LPS-defective insertion mutant strain AP82. Approximately 10⁸ PFU of Ba1 c1 phage were mixed with 10¹⁰ CFU of strain AP82 and 10⁹ or 10¹⁰ CFU of the Ba1-sensitive strain 197N. At the times indicated, a portion of the mixture was withdrawn; the bacteria and bacterium-bound phage were removed by centrifugation, and the titer of the supernatant was determined for unadsorbed phage as described in the text. The graph illustrates the results from a single representative experiment. (B) Adsorption of Ba1 c1 to strain 197N and to each of the phage-resistant mutants according to the protocol described in the text. Values on the y axis represent the proportion of the phage removed by the parent and each mutant after 1 h, normalized to the number of cells added to each assay. The multiplication factor (10¹²) is present to adjust the scale for legibility. The mean error bars are shown for the results of two independent experiments.