FIG. 1.

Analysis of cellular levels of NADPH oxidase components following exposure to A. phagocytophila. HL-60 culture cells or human neutrophils were infected with A. phagocytophila (HGE) for 7 days or 30 min, respectively. Cell lysates (25 μg) of infected and uninfected cells were subjected to SDS-PAGE and Western immunoblotting and probed with antibodies against gp91^phox (rabbit), p67^phox, p47^phox, p40^phox, p22^phox, and α-tubulin. Results shown are representative of three independent experiments.