FIG. 2.

Effects of lysosomal acidification inhibitors on A. phagocytophila-induced p22^phox removal. Human neutrophils were incubated for 30 min at 37°C in HBSSd containing either 300 μM chloroquine or 100 nM concanamycin A prior to 30 min of incubation with host-cell-free A. phagocytophila (HGE). Cell lysates (25 μg/sample) were separated on a polyacrylamide gel, transferred to a nitrocellulose membrane, and probed with anti-p67^phox, -p47^phox, -p40^phox, or -p22^phox antibody. Results are representative of two independent experiments, each with neutrophils derived from different donors and freshly prepared host-cell-free A. phagocytophila.