TABLE 1.
Primary antibodies used to label A. phagocytophila, NADPH oxidase components, or α-tubulin in human peripheral blood neutrophils and HL-60 cells
| Antigen | Origin | Dilution | Source |
|---|---|---|---|
| A. phagocytophila (BDS strain) | Horse | 1:100a (serum) | J. E. Madigan, University of California, Davis |
| gp91phox (C-15) | Goat | 1:100,b 1:1,000b (purified goat IgG in PBS) | Santa Cruz Biotechnology, Inc., Santa Cruz, Calif. |
| gp91phox C-terminal aa 536-555 | Rabbit | 1:1,000b (serum) | S. Tsunawaki, National Children's Medical Research Center, Tokyo, Japan |
| p22phox C-terminal aa 177-195 | Rabbit | 1:100,a 1:1,000b (serum) | S. Tsunawaki |
| p47phox C-terminal aa 376-390 | Rabbit | 1:100,a 1:1,000b (serum) | S. Tsunawaki |
| p40phox N-terminal aa 1-15 | Rabbit | 1:1,000b (serum) | S. Tsunawaki |
| p67 phax C-terminal aa 317-469 | Mouse | 1:100,a 1:1000b (IgG2b in PBS) | Transduction Laboratories, Lexington, Ky. |
| Rac2 (C-11) | Rabbit | 1:100a (purified rabbit IgG in PBS) | Santa Cruz Biotechnology, Inc. |
| α-tubulin | Mouse | 1:1,000b (ascitic fluid in PBS) | Cedarlane, Hornby, Ontario, Canada |
a
Dilution used for double immunofluorescence labeling.
b
Dilution used for Western immunoblotting.