TABLE 2.

Effects of MDC, genistein, neomycin, and H89 treatment on A. phagocytophila internalization in human peripheral blood neutrophils

Treatmenta	% Infected cellsb
None	7.0 ± 1.7
MDC	0.9 ± 0.4
Genistein	1.6 ± 0.6
Neomycin	0.3 ± 0.6
H89	0.7 ± 1.2

^aNeutrophils preincubated for 30 min with inhibitors were further incubated with host cell-free A. phagocytophila in the presence of each inhibitor for 2 h at 37°C. The cells were treated with 2 mg of pronase/ml to remove uninternalized A. phagocytophila and cultured in the absence of inhibitors for 16 h at 37°C, and infection rates were determined.

^bMeans ± standard deviations (n = 3). Results are representative of three independent experiments.