TABLE 3.
Inhibition of O2− release by human peripheral blood neutrophils in response to PMA by A. phagocytophila in the presence of MDC, neomycin, genistein, H89, and anti-PSGL-1
| Treatmenta | Reduction of ferricytochrome c (nmol of O2−/106 neutrophils)b |
|---|---|
| Experiment 1 | |
| Medium control | 1.8 ± 0.2 |
| PMA | 21.1 ± 3.4∗ |
| MDC | 4.5 ± 0.4 |
| MDC + PMA | 18.7 ± 0.3∗ |
| MDC + A. phagocytophila + PMA | 4.7 ± 0.3 |
| Neomycin | 2.3 ± 0.4 |
| Neomycin + PMA | 14.7 ± 0.2∗ |
| Neomycin + A. phagocytophila + PMA | 2.8 ± 0.5 |
| Genistein | 3.7 ± 2.2 |
| Genistein + PMA | 15.4 ± 1.2∗ |
| Genistein + A. phagocytophila + PMA | 4 ± 3.1 |
| H89 | 3.8 ± 1.4 |
| H89 + PMA | 16.4 ± 0.3∗ |
| H89 + A. phagocytophila + PMA | 2.4 ± 0.1 |
| Experiment 2 | |
| Medium control | 0.4 ± 0.6 |
| PMA | 8.5 ± 0.6∗ |
| A. phagocytophila | 1.7 ± 0.2 |
| A. phagocytophila + PMA | 1.9 ± 1.0 |
| PL1 + PMA | 6.3 ± 0.6∗ |
| A. phagocytophila + PL1 + PMA | 1.8 ± 0.5 |
a
Neutrophils were pretreated with or without reagents (250 μM MDC, 10 μM neomycin, 50 μM genistein, 10 μM H89, or 2.5 pg of anti-PSGL-1) for 30 min prior to addition of A. phagocytophila and PMA stimulation. Reagents were present throughout assays.
b
Mean ± standard deviation (n = 3). Results are representative of more than three independent experiments. ∗, P < 0.01 compared to neutrophil-alone (no PMA) control (Student's t test).