Comparison of the reduced and oxidized forms of mMICAL489.(A and B) Superposition of the two forms. The FAD molecules are drawn as balls and sticks (carbons of oxidized mMICAL489, cyan; carbons of reduced mMICAL489, orange). The main chain of the oxidized form is depicted as a ribbon. B is rotated by 90° about the x axis relative to A. (C and D) Coordination of the isoalloxazine ring in the oxidized (C) and reduced (D) forms viewed from a common orientation. The isoalloxazine ring and selected residues are depicted as sticks (orange, carbon of reduced isoalloxazine; gray, protein carbon), waters are shown as spheres, and H bonds are shown as yellow dashes.