TABLE 5.

Heterotypic protection of mice after oral immuization and challenge infection with various serotypes of S. pneumoniae

Pneumococcal serotype	Vaccine	CFU/ml (mean ± SEM) from:
		Bronchoalveoli	Blood
4	Mock	(4 ± 1.6) × 107	(7 ± 0.5) × 107
	nPsaA−CTB	(3 ± 2.0) × 104 (0.01)a	(5 ± 1.6) × 103 (<0.001)
	e(PsaA−CTB)1	(6 ± 1.0) × 102 (0.01)	(3 ± 1.4) × 103 (<0.001)
6B	Mock	(2 ± 0.8) × 105	(4 ± 3.0) × 104
	nPsaA−CTB	(3 ± 2.3) × 104 (<0.05)	(3 ± 1.8) × 103 (NSb)
	e(PsaA−CTB)1	(3 ± 1.9) × 10 (0.03)	(2 ± 1.6) × 102 (BSc)
14	Mock	(3 ± 1.2) × 107	(3 ± 0.7) × 103
	nPsaA−CTB	(1 ± 0.5) × 106 (0.01)	(4 ± 1.1) × 102 (0.004)
	e(PsaA−CTB)1	(8 ± 3.6) × 104 (0.01)	(1 ± 0.7) × 102 (0.002)
19F	Mock	(4 ± 0.9) × 103	(9 ± 1.8) × 102
	nPsaA−CTB	(6 ± 2.9) × 102 (<0.001)	(1 ± 0.7) × 10 (<0.001)
	e(PsaA−CTB)1	(1 ± 0.6) × 102 (<0.001)	(3 ± 01.8) × 1 (<0.001)
23F	Mock	(2 ± 1.1) × 108	(1 ± 0.3) × 104
	nPsaA−CTB	(3 ± 2.4) × 105 (0.04)	(1 ± 1.2) × 103 (0.002)
	e(PsaA−CTB)1	(2 ± 1.0) × 103 (0.04)	(2 ± 1.9) × 10 (<0.001)

^aThe numbers in parentheses indicate statistical significance (P value) of the difference between the mock-immunized mice and test group of mice.

^bNS, not significant (P ≥ 0.1).

^cBS, borderline significance (0.05 < P ≤ 0.1).