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DNA fragmentation patterns in Ramos Burkitt's lymphoma B cells exposed to Stx1 or Stx2 holotoxins or their cloned B subunits for 18 h at 37°C. Fragmented DNA was isolated and precipitated from cell lysates and analyzed by agarose gel electrophoresis by using a 2% gel. Lane 1, 1-kb DNA ladder; lane 2, no treatment; lane 3, 5 μM camptothecin; lane 4, 1 ng of Stx1/ml; lane 5, 1 ng of Stx2/ml; lane 6, 10 μg of Stx1 B subunit/ml; lane 7, 10 μg of Stx2 B subunit/ml.
