TABLE 3.
Classification and strain distribution of loci of the FCT region
| Locus | Category | No. of aaa residues | (Putative) function | COGnitor search results | BlastP search resultsb
|
Distribution of loci in FCT region ofc:
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Best alignment | BlastE value | % aa identity (total no. of residues) | SF370 (M1) | Manfredo (M5) | D471 (M6) | A735(M12) | CS101/B737 (M49) | |||||
| hsp33 (SPy0123) | 1 | 264 | Heat shock protein | COG1281 | Chaperonin SP2188 (S. pneumoniae) | e-110 | 75 (264) | + | + | + | + | + |
| SPy0136 | 1 | 221 | Unknown | None | NA | Above cutoff | NA | + | + | + | + | + |
| rofA (SPy0124) | 2 | 439 | Transcriptional regulator | None | Nra (S. pyogenes) | e-148 | 64 (441) | + | − | + | + | − |
| nra | 2 | 511 | Transcriptional regulator | None | RofA (S. pyogenes) | e-160 | 61 (499) | − | + | − | − | + |
| lepAL | 2 | 173 | Signal peptidase I | COG0681 | SPy0127 (S. pyogenes) | 2e-23 | 44 (132) | − | + | − | + | + |
| etfLSL (B) (3′ portion) | 2 | 241 | Unknown | None | SPy0129 (S. pyogenes) | 5e-64 | 46 (240) | − | + | − | + | + |
| orf2 | 2 | 195 | Unknown | None | SPy0130 (S. pyogenes) | 3e-14 | 29 (123) | − | + | − | + | + |
| msmRLd | 2 | 401 | AraC-type DNA-binding domain-containing protein | COG2027 | AraC-like activator (S. agalactiae) | 3e-12 | 31 (164) | − | + | − | + | + |
| SPy0135 | 2 | 227 | Fimbria-associated protein (putative sortase) | None | Putative sortase SP0467 (S. pneumoniae) | 2e-44 | 50 (178) | + | − | + | + | − |
| prtF/prtF1/sfbI | 3 | Varies | Fn-binding protein | None | Cpa (S. pyogenes) | 1e-65 | NA | − | − | + | + | − |
| cpa (SPy0125) | 3 | Varies | Collagen-binding protein | None | Protein F (S. pyogenes) | 2e-20 | NA | + | + (partial) | − | + | + |
| prtF2/pfbpI | 3 | Varies | Fn-binding protein | None | Protein F; opacity factor (S. pyogenes) | 1e-21; 1e-16 | NA | − | + | − | + | + |
| etfLSL (A) (5′ portion) | 3 | Varies | Unknown | None | SPy0128 (S. pyogenes) | 5e-57 | 43 (240) | − | + | − | + | + |
| SPy0127 (lepAL.1) | 4 | 185 | Signal peptidase I | COG0681 | LepAL (S. pyogenes) | 1e-30 | 43 (171) | + | − | − | − | − |
| SPy0128 | 4 | 340 | Unknown | None | EtfLSL (S. pyogenes) | 4e-41 | 35 (355) | + | − | − | − | − |
| SPy0129 | 4 | 237 | Unknown | None | EtfLSL (S. pyogenes) | 1e-60 | 46 (240) | + | − | − | − | − |
| SPy0130 | 4 | 215 | Unknown | None | Orf2 (S. pyogenes) | 1e-14 | 29 (173) | + | − | − | − | − |
| SPy0131 | 4 | 450 | Unknown | None | Unknown (Pseudomonas) | 7e-50 | 32 (426) | + | − | − | − | − |
| SPy0133 | 4 | 116 | Transposase | COG3436 | IS 66 family element (S. pneumoniae) | 1e-46 | 77 (116) | + | − | − | − | − |
| fctX | 4 | 1,036 | Unknown | None | Cell wall surface anchor family SP0462 (S. pneumoniae) | 3e-6 | 24 (392) | − | − | + | − | − |
| tee6 | 4 | 537 | Unknown | None | NA | Above cutoff | NA | − | − | + | − | − |
a
aa, amino acid.
b
Reported using cutoff E values at e-4. For category 3 loci only, values are reported for best alignment to another locus within S. pyogenes. All other values are based on highest score with entire National Center for Biotechnology Information database (all species). NA, not applicable.
c
SPy0135 in A735 contains a disrupted ORF (stop codon; frameshift). The eftLSL 5′ portion of CS101/B737 is a separate ORF in Manfredo and A735; all share stretches of high nucleotide identity at their 5′ and 3′ ends. +, present; −, absent.
d
Composite of strains CS101 and B737.