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Intracellular replication rates of MC 8013.6 (▿) and MC 8013.6 hmbR::Kan (□) as determined by gentamicin protection assay. Ferric nitrate was included in the invasion media (T = −13 to −1 in Fig. 1) to allow efficient growth and entry of the mutant. MC 8013.6 hmbR::Kan replicated faster than wt MC 8013.6 within cells. The datum points represent the averages of three or more cultures, with error bars representing the standard deviation.
