TABLE 7.
Effect of OPN genotype on B. burgdorferi-specific IgG isotypes in sera from OPN-deficient micea
| Mouse strain | IgG1 | IgG2a | IgG2b | IgG3 |
|---|---|---|---|---|
| C57 × 129S-Opn+/+b | 12.8 ± 3.4 | NDc | 2,249 ± 672 | 1,294 ± 453 |
| C57 × 129S-Opn−/−b | 13.3 ± 9.0 | ND | 2,272 ± 392 | 1,098 ± 496 |
| 129S-Opn+/+ | 41.2 ± 11.1 | 482.7 ± 197.8 | 3,065 ± 827 | 1,499 ± 1,337 |
| 129S-Opn−/− | 53.3 ± 43.7 | 585.7 ± 157.4 | 2,744 ± 534 | 1,311 ± 954 |
a
Mice were infected with 2,000 B. burgdorferi bacteria. Serum collected at 4 weeks postinfection was assessed by ELISA for IgG content as described in Materials and Methods. Values represent levels of Ig in nanograms per milliliter. Results are presented as arithmetic means ± standard deviations.
b
C57BL/6J × (C57BL/6J × 129S)-Opn background.
c
ND, not determined.
d
All samples had detectable IgG of each isotype.