FIG. 4.

Effect of MAb 1C3 on recombinant chitinase activity in vitro. MAb 1C3 or IgG2b isotype MAbs were incubated with either rPfCHT1 or rPgCHT1 and chitinase activity measured by using 4-MU-chitotrioside as the substrate. Error bars represent the standard deviations. Excitation was determined at 365 nm and emission at 460 nm to determine the relative fluorescent units (chitinase activity). The reduction of rPfCHT1 chitinase activity when treated with MAb 1C3 was statistically significant, while there was no significant effect of isotype control antibodies on rPfCHT1 or rPgCHT1, and no effect of MAb 1C3 on rPgCHT1.