FIG. 2.

IL-10 kinetics and dose response after stimulation with GLP. LPS was used as positive control. PBMC were stimulated with either S. enterica serovar Abortus Equi LPS or L. interrogans serovar Copenhageni GLP in the indicated doses and incubated for 72 h. Supernatants were collected at the specified time points, and TNF-α was measured by ELISA. Results are means ± SDs from two experiments. Closed squares. control; closed diamonds, LPS at 1 μg/ml; open triangles, GLP at 0.3 μg/ml; open squares, GLP at 0.03 μg/ml; open diamonds, GLP at 0.003 μg/ml. For all GLP stimuli compared with controls, P = 0.05; ∗, P < 0.05 compared to control.