TABLE 2.
IFN-γ production in spleen cell cultures from i.g. immunized mice
| Antigen | IFN-γ concn (pg/ml) in cells from mice receivinga:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| O:3 | O:3(pCI) | O:3(pCIBFR) | O:3(pCIP39) | O:9 | O:9(pCI) | O:9(pCIBFR) | O:9(pCIP39) | |
| RPMI | — | — | — | — | — | — | — | — |
| BFR | 125 | 125 | 1,700 | — | 62.5 | 75 | 1,970 | — |
| P39 | — | — | — | 1,900 | 75 | — | — | 2,100 |
| O:3 | 2,200 | 2,200 | 1,900 | 2,000 | ND | ND | ND | ND |
| O:9 | ND | ND | ND | ND | 1,800 | 1,980 | 2,200 | 2,000 |
| ConA | 3,200 | 3,300 | 1,980 | 2,100 | 2,200 | 2,450 | 2,300 | 2,500 |
a
Splenocytes were recovered from groups of four mice 14 days after two inoculations. Levels of IFN-γ in culture supernatants from triplicate wells were measured after 92 h of incubation. ND, not determined; —, below the detection limit of the assay (40 pg/ml).