TABLE 3.
Protection against a B. abortus 544 challenge provided to BALB/c mice by vaccination with live O:3, O:9, or S19 strainsa
Treatment group (n = 3 or 4) | Vaccine injection route | Vaccine | Vaccination challenge delay (wk) | Log10 brucellae in spleen (mean ± SD) at time (wk) postchallenge
|
Log units of protection at wk
|
||
---|---|---|---|---|---|---|---|
4 | 8 | 4 | 8 | ||||
1 | i.g. | O:3 | 2 | 4.32 ± 0.17 | 3.58 ± 0.13 | 0.13 | 0.09 |
2 | i.g. | O:3(pCI) | 2 | 4.43 ± 0.14 | 3.55 ± 0.02 | 0.02 | 0.12 |
3 | i.g. | O:3(pCIBFR) | 2 | 3.96 ± 0.12 | 2.95 ± 0.16 | 0.49 | 0.72 |
4 | i.g. | O:3(pCIP39) | 2 | 3.76 ± 0.20 | 2.80 ± 0.11 | 0.69 | 0.87b,c |
5 | i.g. | O:9 | 2 | 3.02 ± 0.16 | 2.52 ± 0.16 | 1.43b,c | 1.15b,c |
6 | i.g. | O:9(pCI) | 2 | 3.15 ± 0.19 | 2.44 ± 0.13 | 1.3b,c | 1.23b |
7 | i.g. | O:9(pCIBFR) | 2 | 2.81 ± 0.45 | 2.04 ± 0.10 | 1.64b | 1.63b |
8 | i.g. | O:9(pCIP39) | 2 | 2.03 ± 0.25 | 1.7 | 2.22b | 1.97b |
9 | i.g. | O:3-O:9 | 2 | 2.92 ± 0.07 | 2.40 ± 0.12 | 1.53b,c | 1.27b |
10 | i.g. | O:3(pCIP39)-O:9 | 2 | 2.35 ± 0.02 | 1.7 | 2.1b | 1.97b |
11 | i.g. | O:3 | 8 | 4.27 ± 0.13 | 3.61 ± 0.02 | 0.18 | 0.06 |
12 | i.g. | O:3(pCIBFR) | 8 | 4.15 ± 0.07 | 3.42 ± 0.04 | 0.30 | 0.25 |
13 | i.g. | O:3(pCIP39) | 8 | 4.03 ± 0.17 | 3.26 ± 0.03 | 0.42 | 0.41 |
14 | i.g. | O:9 | 8 | 3.19 ± 0.04 | 2.03 ± 0.16 | 1.26b,c | 1.64b |
15 | i.g. | O:9(pCIBFR) | 8 | 3.08 ± 0.04 | 1.97 ± 0.18 | 1.37b,c | 1.7b |
16 | i.g. | O:9(pCIP39) | 8 | 2.7 ± 0.25 | 1.72 ± 0.14 | 1.75b | 1.92b |
17 | i.g. | B19 | 4 | 3.67 ± 0.19 | 2.77 ± 0.22 | 0.73b,c | 0.9b,c |
18 | i.p. | B19 | 4 | 2.15 ± 0.15 | 1.7 | 2.3b | 1.97b |
19 | i.p. | B19 | 8 | 1.85 ± 0.15 | 1.7 | 2.6b | 1.97b |
20 | i.p. | PBS | 4.45 ± 0.13 | 3.67 ± 0.08 |
Groups 1 through 16 were vaccinated with 1 × 108 to 3 × 108 CFU, group 17 was vaccinated with 1 × 109 CFU, and groups 18 and 19 were vaccinated with 5 × 104 CFU. All groups were challenged i.p. with 5 × 104 CFU of B. abortus 544.
Statistically different from the unvaccinated control (P = 0.05).
Statistically different from the B19 i.p. vaccinated control (P = 0.05).