Skip to main content
. 2002 Apr;70(4):1783–1790. doi: 10.1128/IAI.70.4.1783-1790.2002

FIG. 2.

FIG. 2.

Thin-layer chromatography of spent culture supernatant and patient sputum extracts. Samples were applied to a reverse-phase C18 plate and developed as outlined, and autoinducers were detected by overlaying with a suspension of A. tumefaciens A136(pCF218)(pCF372). Samples in lanes 1 to 10 are C6-HSL (3 nmol), C8-HSL (3 nmol), P. aeruginosa PAO1 supernatant, P. aeruginosa CF isolate 4384 supernatant, P. aeruginosa CF isolate 14655 supernatant, patient 3 sputum extract, B. cepacia K56-2 supernatant, P. aeruginosa CF isolate 4384 supernatant, P. aeruginosa CF isolate 6106 supernatant, and P. aeruginosa CF isolate 14655 supernatant, respectively. Putative identification of spots in samples (from bottom to top): for PAO1 and 4384, 3-oxo-C12-HSL, 3-oxo-C10-HSL, 3-oxo-C8-HSL, and 3-oxo-C6-HSL; for 14655, C8-HSL and 3-oxo-C8-HSL; for sputum samples, 3-oxo-C12-HSL, 3-oxo-C10-HSL, and 3-oxo-C8-HSL; for B. cepacia, K56-2, C8-HSL and C6-HSL.