TABLE 3.
C4-HSL and 3-oxo-C12-HSL in sputum samplesa
Sample no. | Concn (nM)
|
|
---|---|---|
C4-HSL | 3-Oxo-C12-HSL | |
330 | 0.00 | 1.86 |
342 | 0.00 | 1.39 |
351 | 5.00 | 14.40 |
360 | 1.13 | 1.64 |
361 | 2.61 | 2.15 |
362 | 0.84 | 21.20 |
363 | 0.00 | 1.06 |
364 | 1.84 | 0.00 |
365 | 1.00 | 0.00 |
366 | 0.00 | 14.70 |
11938 | 0.00 | 1.13 |
13165 | 0.00 | 0.92 |
Concentrations were determined by comparing β-galactosidase levels obtained from known amounts of synthetic 3-oxo-C12-HSL or C4-HSL using reporter plasmids pKDT17 and pECP61.5.