FIG. 4.

Immunoblot analysis of flagellin protein in wild-type and flagellar mutant strains. Equal amounts of protein from whole-cell or supernatant fractions were separated on SDS-12.5% PAGE gels and immunoblotted with anti-flagellin antibody as described in Materials and Methods. (A) Wild-type strain J2315, D9 (fliG::mini-Tn5Tc), and CM340 (D9 complemented with fliG). (B) Wild-type strain J2315, CM58 (fliI::cat), and CM100 (CM58 complemented with fliI). The molecular masses of the protein standards are indicated on the left. The position of the 45-kDa flagellin protein band is indicated by an arrow.