FIG. 1.
Identification of multiple vsp-related genomic fragments in M. bovis strains. Four micrograms of chromosomal DNA of each strain was digested with the HindIII restriction enzyme, subjected to Southern blot hybridization, and probed with the 32P-labeled vspA gene (A), with oligonucleotide RE1 (B), and with oligonucleotide RF2 (C). The conditions used for oligonucleotide labeling, DNA labeling, and Southern blot hybridization have been described elsewhere (9, 10). The M. bovis strains used included type strain PG45 (lane 1), D490 (lane 2), D857 (lane 3), D860 (lane 4), D1098 (lane 5), D1499 (lane 6), D1517 (lane 7), and 422 (lane 8). The positions of molecular size markers are indicated on the left.