TABLE 1.
The PhoP-PhoS homologs identified in E. faecalis V583 and outcome of disruption in E. faecalis OG1RF
| Locus name | E value (R/S) | Directiona | Oligonucleotidesb | Disruption outcomec | Best hit (R/S)d |
|---|---|---|---|---|---|
| 10276 | 5e-75/4e-05 | R→S | 5′ TTGGAAAAAGAAGGCTATC | Viable | Response regulator of Listeria innocua/sensor kinase of Listeria monocytogenes; NP_471974/NP_466023 |
| 5′ ACGTAAATGTCTAATGTGGAC | |||||
| 10299 | 3e-68/2e-26 | R→S | 5′ AAAATGGATGGCTTAGAA | Not recovered | VicR/VicK of Enterococcus faecalis; CAB64972/CAB64973 |
| 5′ AAATACGTTGGATGACTTG | |||||
| 10275 | 9e-49/3e-19 | R→S | 5′ ATGATGATAAAGAAATTGTAGAAC | Viable | Response regulator/histidine kinase of Lactobacillus sakei; AAD10265/AAD10266 |
| 5′ CACACGATTTGGATGACT | |||||
| 10292 | 2e-46/1e-20 | R→S | 5′ TAAAGATGATGGTAAAGAAGG | Viable | Response regulator/histidine kinase of Lactobacillus sakei; AAD10267/AAD10268 |
| 5′ ATATGGGCATCAACTGTC | |||||
| 10293 | 1e-41/2e-82 | R→S | 5′ AGATGAAAAGAACTTAGCGAGATT | Viable | LisR of Listeria monocytogenes/sensor kinase of Lactococcus lactis; AAF03932/NP_267749 |
| 5′ CGTTCACGTTTTCCATTAGC | |||||
| 10321e | 5e-41/1e-11 | R→S | NA | NA | VanRB/VanSB of Enterococcus faecalis; Q47744/Q47745 |
| 10303 | 3e-38/2e-25 | S→R | 5′ TGCCCAAAAAGAAAATCCAT | Viable | Response regulator/histidine kinase of Clostridium acetobutylicum; NP_346962/NP_346958 |
| 5′ AAGCGTTCGAATAAATAAGGTAAT | |||||
| 10288f | 2e-37/6e-10 | S→R | 5′ TTAATAAAACTGCTGAACCA | NA | Response regulator/sensor kinase of Listeria innocua; NP_472154/NP_472155 |
| 5′ TCCATTTTACCATTGTGC | |||||
| 37200 | 4e-30/8e-25 | S→R | 5′ AAATATGAAGCTAATCGGAAAGAA | Viable | Response regulator/histidine kinase of Clostridium acetobutylicum; NP_346962/NP_346958 |
| 5′ AAGCGTTCGAATAAATAAGGTAAT | |||||
| 10298 | 6e-27/5e-07 | R→S | 5′ CGAGAATTAATTAGCGAGGAA | Viable | Response regulator/histidine kinase of Lactobacillus sakei; AAD10258/AAD10259 |
| 5′ CCGTCAGCGTATTGTCATC | |||||
| 10285f | 2e-26/3e-06 | R→S | Could not amplify | NA | Response regulator VncR/sensor kinase VncS of Streptococcus pneumoniae; NP_345115/NP_345116 |
a
Direction of transcription. In all cases, the gene on the left was disrupted. R, response regulator; S, sensor kinase.
b
Oligonucleotides used to amplify the gene on the left. NA, not attempted.
c
The outcome of gene disruption.
d
The GenBank accession numbers are given.
e
The vancomycin resistance regulatory system VanRBSB.
f
PCR product not obtained from OG1RF.