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Purification of RCP from P. gingivalis
| Step | Total protein (mg) | Total activity (U) | Vol (ml) | Sp acta | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Cell extraction | 319.3 | 12.2 | 103.0 | 0.04 | 100 | 1.0 |
| DEAE-Sephacel column chromatography | 19.4 | 9.7 | 45.0 | 0.5 | 80 | 12.5 |
| DEAE-5PW HPLC | 1.0 | 2.0 | 4.8 | 2.0 | 16 | 50.0 |
| 1st TSK G2000SWXL HPLC | 0.5 | 1.2 | 3.0 | 2.4 | 10 | 60.0 |
| 2nd TSK G2000SWXL HPLC | 0.2 | 0.7 | 1.2 | 3.5 | 6 | 87.5 |
Specific activity is expressed as units per milligram of protein.
