FIG. 2.
M. bovis BCG inhibits the development of cytolytic CD8+ T-cell response to L. monocytogenes in a dose-dependent manner. BALB/c mice were injected with PBS or with 104 M. bovis BCG or 105 M. bovis BCG. Thirty days later mice were injected with L. monocytogenes (LM). On day 37, pooled spleen cells from various groups of mice were restimulated with irradiated pHem3.3 cells (expressing LLO91-99) for 5 days in the presence of IL-2 (0.1 ng/ml) as described in the Materials and Methods section. Cells were harvested and washed, and effectors were tested for their cytolytic activity on 51Cr-labeled P815 (open symbols) or P815+LLO91-99 (solid symbols) target cells. Means ± standard deviations for triplicate cultures are shown.