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. 2002 Apr;70(4):1896–1908. doi: 10.1128/IAI.70.4.1896-1908.2002

TABLE 1.

Characteristics of φP27 ORFs and deduced proteins

ORF label Gene name Directionb Left end position no. Right end position no. No. of amino acids Function of deduced protein e value % Identitya Accession no. and sequence similarityc
attL 1 11
L01 int − 30 1343 437 Integrase 0.0 70 (All) BAB19626; putative integrase, prophage VT1-Sa (E. coli)
0.0 70 (All) AAG57258; putative integrase, prophage CP-933V (E. coli)
L02 xis − 1399 1635 78 Excisionase 3e−16 53 (66) NP_049462; putative excisionase, phage 933W (E. coli)
3e−16 53 (66) NP_050501; putative excisionase, prophage VT2-Sa (E. coli)
L03 − 1794 2159 121 1e−22 63 (80) BAB36434; hypothetical protein (E. coli RIMD 0509952)
2e−13 60 (60) AAG57256; hypothetical protein, prophage CP-933V (E. coli)
L04 − 2068 2511 147 7e−04 34 (100) NP_050134; hypothetical protein, prophage φadh (Lactobacillus gasseri)
L05 − 2480 3307 275 Protease e−124 79 (All) NP_047914; putative serine protease (Y. pestis)
L06 − 3669 4307 212
L07 − 4247 4450 67
L08 − 4632 4997 121
L09 + 5106 5285 59 5e−06 78 (28) AAG55461; hypothetical protein, prophage CP-933M (E. coli)
L10 − 5575 5991 138 Repressor 1e−06 37 (69) AAC74643; DicA repressor (E. coli)
L11 cI − 6095 6751 218 CI repressor 1e−45 42 (All) NP_059606; repressor C2, phage P22 (S. enterica)
L12 + 6863 7090 75
L13 − 7104 7775 223
L14 roi + 7876 8583 235 DNA binding 1e−32 55 (136) NP_112063; DNA-binding protein Roi, phage HK620 (E. coli)
5e−32 52 (140) AAD04652; Roi, phage H-19B (E. coli)
L15 + 8636 9460 274 Nucleic acid binding 5e−20 31 (194) B82549; hypothetical protein XF2506 (X. fastidiosa)
5e−14 33 (127) AAG55918; putative antirepressor, prophage CP-933N (E. coli)
8e−14 38 (118) NP_046925; antirepressor AntB, phage N15 (E. coli)
L16 + 9457 10116 219 9e−31 48 (153) S34345; hypothetical protein 179 (Shigella flexneri)
1e−12 43 (112) AAG54596; putative regulator, prophage CP-933I (E. coli)
L17 + 10334 11260 308 DNA binding 9e−31 42 (196) AAK16983; hypothetical protein, prophage CP-933P (E. coli)
2e−28 33 (227) AAG55470; hypothetical protein, prophage CP-933M (E. coli)
L18 P + 11391 12149 252 DNA replication 2e−24 31 (207) BAB12748; DNA replication protein DnaC (Buchnera aphidicola)
1e−21 27 (210) AAG55471; DNA replication factor, prophage CP-933M (E. coli)
L19 + 12146 13537 463 Helicase 6e−87 39 (447) NP_059611; helicase, phage P22 (S. enterica)
2e−86 39 (451) NP_037740; gp55, prophage HK97 (E. coli)
L20 + 13549 13791 80
L21 Q + 13791 14171 126 Late antiterminator 6e−25 50 (119) AAG55890; Q antiterminator, prophage CP-933N (E. coli)
7e−24 47 (120) O48429; antitermination protein Q, prophage H-19B (E. coli)
3e−23 44 (126) CAB39299; antitermination protein Q, phage 933W (E. coli)
L22 + 14266 15495 409 6e−04 32 (127) CAA22431; putative chromatin assembly factor (Schizosaccharomyces pombe)
L23 + 15711 15908 65 5e−29 98 (63) AAG56133; hypothetical protein, prophage CP-933O (E. coli)
4e−28 96 (63) BAB35615; hypothetical protein (E. coli RIMD 0509952)
1e−19 97 (49) AAG55892; hypothetical protein, prophage CP-933N (E. coli)
L24 dam + 16059 17117 352 DNA methylase 0.0 86 (All) AAG56134; adenine methyltransferase, prophage CP-933O (E. coli)
0.0 86 (All) BAB35203; DNA methylase (E. coli RIMD 0509952)
2e−81 55 (282) NP_046948; adenine-specific methylase, phage N15 (E. coli)
ileZ + 17158 17233 tRNA
arqO + 17335 17411 tRNA
L25 stxA2e + 17502 18461 319 rRNA N-glycosidase 0.0 99 (All) CAA57173; Stx2e, A subunit (E. coli)
L26 stxB2e + 18474 18737 87 Receptor binding 3e−44 100 (All) CAA57176; Stx2e, B subunit (E. coli)
L27 − 18788 18985 65 2e−28 96 (61) CAC05562; hypothetical protein (E. coli T4/97)
5e−25 96 (55) CAC05572; hypothetical protein (E. coli H.I.8)
L28 S + 19114 19548 144 Holin 8e−06 36 (86) AAF80841; ORF 89, prophage D3 (Pseudomonas aeruginosa)
0.002 28 (123) BAA36235; holin ORF 9, phage φCTX (P. aeruginosa)
L29 S + 19538 19813 91 Holin 0.005 34 (85) H83531; hypothetical protein PA0909 (P. aeruginosa)
0.049 32 (86) BAA36236; holin ORF 10, phage φCTX (P. aeruginosa)
L30 R + 19816 20193 125 Endolysin 3e−25 48 (All) CAA09701; endolysin gp 19, phage PS3 (S. enterica serovar Typhimurium)
3e−12 38 (119) AAC38580; peptidoglycan lytic enzyme (Listeria monocytogenes)
L31 + 20136 20351 71
L32 + 20630 20785 51
L33 + 20985 21332 115
L34 + 21394 21744 116 1e−37 62 (All) CAB58450; hypothetical protein ORF 7 (Xenorhabdus nematophilus)
2e−11 39 (111) AAB59284; putative holin, prophage φ105 (B. subtilis)
cos 21806 21815
L35 + 21860 22330 156 Terminase small subunit 2e−11 31 (138) AAG50266; hypothetical protein, phage GMSE-1 (Sodalis)
2e−07 31 (96) AAG55950; hypothetical protein, prophage CP-933C (E. coli)
2e−07 31 (96) BAB35020; terminase small subunit (E. coli RIMD 0509952)
L36 + 22345 24057 570 Terminase large subunit e−178 77 (293) P75978; hypothetical protein YmfN (E. coli)
e−141 45 (All) NP_061498; terminase, phage D3 (P. aeruginosa)
L37 + 24069 24251 60 2e−27 95 (All) P75979; hypothetical protein YmfR (E. coli)
L38 + 24251 25492 413 Portal protein 5e−63 84 (135) P75980; hypothetical protein YmfO (E. coli)
4e−52 33 (372) NP_108600; head portal protein (M. loti)
1e−44 32 (375) NP_037699; portal protein, phage HK97 (E. coli)
L39 + 25470 26120 216 Prohead protease 2e−24 40 (172) AAF13181; putative prohead protease (Rhodobacter capsulatus)
2e−18 35 (196) NP_037665; head maturation protease, prophage HK022 (E. coli)
L40 + 26134 27357 407 Major capsid protein 2e−73 36 (All) NP_108602; phage major capsid protein gp36 (M. loti)
2e−58 34 (All) AAF27364; phage φC31 gp36-like protein (H. influenzae)
L41 + 27404 27727 107 6e−04 29 (104) AAG55948; hypothetical protein, prophage CP-933C (E. coli)
L42 + 28067 28630 187
L43 + 28627 29190 187
L44 + 29358 30854 498 Tail sheath protein 5e−78 35 (All) P44233; putative tail sheath protein, prophage FLUMU (H. influenzae)
7e−75 35 (All) NP_050643; sheath protein gpL, phage Mu (E. coli)
L45 + 30854 31210 118
L46 + 31210 31539 109
L47 + 31624 33573 649 Tail length determinator 9e−41 26 (493) F82769; tail protein XF0730 (X. fastidiosa)
3e−13 22 (473) NP_046782; putative tail length determinator gpT, phage P2 (E. coli)
L48 + 33589 34980 463 DNA binding 1e−23 26 (450) P71389; DNA circulation protein, prophage FLUMU (H. influenzae)
3e−8 23 (479) NP_050647; DNA circulation protein N, phage Mu (E. coli)
L49 + 34977 36032 351 4e−40 30 (All) NP_050648; tail protein P, phage Mu (E. coli)
L50 + 36032 36565 177 Baseplate assembly 3e−12 31 (170) AAF41502; putative baseplate assembly protein V (Neisseria meningitidis)
3e−11 26 (174) NP_050649; baseplate assembly protein gp45, phage Mu (E. coli)
L51 + 36571 36984 137 4e−14 40 (130) BAB38409; hypothetical protein (E. coli RIMD 0509952)
8e−11 41 (112) NP_050650; gp46, phage Mu (E. coli)
L52 + 36977 38059 360 3e−78 55 (260) P75981; hypothetical protein YmfP, prophage E14 (E. coli)
1e−29 31 (330) NP_050651; gp47, phage Mu (E. coli)
L53 + 38059 38649 196 2e−59 55 (194) P75982; hypothetical protein YmfQ, prophage E14 (E. coli)
1e−05 26 (182) NP_050652; gp48, phage Mu (E. coli)
L54 + 38636 39634 332 Tail fiber protein 2e−33 39 (247) A42463; hypothetical protein Bcv (Shigella boydii)
7e−20 31 (305) NP_046775; putative tail fiber protein gpH, phage P2 (E. coli)
L55 + 39637 40185 182 Tail fiber assembly 3e−37 40 (All) C42463; hypothetical protein B177 (S. boydii)
2e−29 36 (All) NP_050654; tail fiber assembly protein U, phage Mu (E. coli)
L56 + 40209 41681 490 Tail fiber protein 2e−20 32 (235) BAB35654; putative tail fiber protein (E. coli RIMD 0509952)
3e−20 32 (235) AAK16943; putative tail fiber protein, prophage CP-933P (E. coli)
L57 + 41678 42244 188 5e−29 35 (All) AAF63233; ORF 191A, prophage P-EibA (E. coli)
L58 + 42399 42575 58
attR 42565 42575
a

Numbers in parentheses represent the whole number of amino acids from which the sequence identity is calculated. All, whole length identity. Empty fields in the table indicate that no homologous sequences were available.

b

−, lower strand; +, upper strand.

c

The GenBank database was used for homolog searches.