FIG. 1.
Reduced expression of Asd protein by deletion of the asd gene promoter region. SDS-PAGE was performed with cell lysates of S. enterica serovar Typhimurium χ4550 with pYA3333 (entire asd gene with Pasd, pBR ori), pYA3334 (entire asd gene with Pasd, pUC ori), pYA3342 (promoterless SD-asd gene, pBR ori), and pYA3341 (promoterless SD-asd gene, pUC ori). Standards are indicated to the left, and Asd protein (39 kDa) is indicated by an arrow. Lanes; 1, pYA3333; 2, pYA3334; 3, pYA3342; 4, pYA3341.