TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Derivation, source, or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| χ6212 | F− λ− φ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | 36 |
| MGN-617 | thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP4 2-Tc::Mu [λpir]; Kmr | 44 |
| S. enterica serovar Typhimurium | ||
| χ3339 | SL1344 hisG | 22 |
| χ4550 | SR-11 gyrA1816 Δcrp-1 ΔasdA1 Δ(zhf-4::Tn10) Δcya-1 | 47 |
| χ4746 | χ3339 nadA540::Tn10 Δ(galE-chl-uvrB)-1005; Tetr | Lab collection |
| χ8499 | hisG Δcrp-28 | χ3339 |
| χ8501 | hisG Δcrp-28 ΔasdA16 | χ8499 |
| χ8554 | hisG ΔasdA16 | χ3339 |
| χ8599 | hisG ΔasdA16 atrB13::MudJ | χ8554 |
| JF2430 | LT2 atrB13::MudJ | 15 |
| S. pneumoniae WU2 | Wild-type virulent, encapsulated type 3 | 6 |
| Plasmids | ||
| pYA3193 | Asd+ vector harboring 1.5-kb C-terminally truncated pspA gene; pUCori | 37 |
| pYA3333 | Asd+; pBRori | Lab collection |
| pYA3334 | Asd+; pUCori | Lab collection |
| pYA3341 | Asd+; pUCori | This study |
| pYA3342 | Asd+; pBRori | This study |
| pYA3493 | pYA3342 derivative β-lactamase signal sequence-based periplasmic secretion plasmid | This study |
| pYA3494 | 0.7-kb DNA encoding the α-helical region of PspA in pYA3493 | This study |
| pYA3496 | 0.7-kb DNA encoding the α-helical region of PspA in pYA3342 for the expression of His-tagged PspA | This study |
| pBR322 | Cloning vector; Apr Tcr | 3 |
| pMEG-443 | Recombinant suicide plasmid to generate Salmonella ΔasdA16 mutant; Apr Cmr | 27 |
| pMEG-493 | Recombinant suicide plasmid to generate Salmonella Δcrp-28 mutant; Apr Cmr | Megan Health Inc. |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.